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pONSY-Lifeact:mCherry

Plasmid

#111876

Purpose

This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Lifeact fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Expression

Mutation

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB

Plasmid

#180197

Purpose

Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-1224

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGB-Co-RHD

Plasmid

#180207

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-581

Promoter

AdhI

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGB-Co-Cterm

Plasmid

#180208

Purpose

Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-RHD

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 542-1224

Promoter

AdhI

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB-Cterm

Plasmid

#180199

Purpose

Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kBCterm

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 542-1224

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pJG01

Plasmid

#213505

Purpose

expresses TdTomato in Capsaspora owczarzaki

Depositor

Joseph Gerdt

Insert

TdTomato

Use

Tags

Expression

Mutation

Promoter

EF1

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKmyc-Exp5

Plasmid

#12552

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

Exportin-5 (XPO5 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

None

Promoter

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

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pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

NONE

Promoter

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only
pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
CytoCKAR

Plasmid

#14870

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NES, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
BKAR

Plasmid

#14875

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
MitoCKAR

Plasmid

#14872

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, TOM20(1-33), and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
NucCKAR

Plasmid

#14869

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
BKAR-T/A

Plasmid

#14876

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
MyrPalm-BKAR

Plasmid

#14878

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pJES102

Plasmid

#135409

Purpose

E. coli 'blaTEM-1 (without signal sequence) that can be used as a reporter

Depositor

Miriam Braunstein

Article

McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

Insert

blaTEM-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Unknown

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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