We narrowed to 10,175 results for: Coli
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Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-lacZ
Plasmid#72948PurposeEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloningDepositorInsertLevel 0 - Bioparts
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_Uricase
Plasmid#182400PurposeBacterial expression of uricaseDepositorInserturicase
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRIS
Plasmid#120424PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3, IS150
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
ExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rssA
Plasmid#89957PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.DepositorInsertrssA gRNA
ExpressionBacterialPromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only