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DoS2

Plasmid

#244467

Purpose

Containing RP4 oriT, RP4 inc and I-SceI enzyme sequence, for eliminating RP4 plasmid and self-cutting.

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

tetR/tetA promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Irp9

Plasmid

#246319

Purpose

Overexpression of a bacterial bifunctional salicylate synthase targeted to the cytosol

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Fd-Irp9

Plasmid

#246318

Purpose

Overexpression of a bacterial bifunctional salicylate synthase, Irp9, targeted to the chloroplast

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Fd-Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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DoS3 plasmid

Plasmid

#244471

Purpose

Containing RP4 oriT and Cas9 module, for eliminating RP4 plasmid.

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

araBAD promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTRNA scPhe-GAA

Plasmid

#211359

Purpose

Transient expression of scPhe-GAA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

S. cerevisiae Phe-GAA tRNA

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pT7RNAPNev-NB1

Plasmid

#216336

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-eGFP nanobody NB1

Depositor

Mark Styczynski

Article

McSweeney et al Sci Adv. 2025 Feb 21;11(8):eado6280. doi

Insert

T7RNAPN-NB1 fusion

Expression

Bacterial

Available Since

July 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-GST-SLC-FNIP2

Plasmid

#189869

Purpose

Mammalian expression of SLC38A9 N-terminus and FNIP2 fusion protein

Depositor

James Hurley

Article

Jansen et al Sci Adv. 2022 Sep 16;8(37):eadd2926. doi

Insert

SLC38A9 N-Termius and FNIP2 fusion protein

Tags

GST

Expression

Mammalian

Mutation

SLC38A9 residues 1-119, 10-residue GS linker

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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CT362

Plasmid

#233679

Purpose

Red light-inducible Cre-C; Cre-230C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT611

Plasmid

#233669

Purpose

Red light-inducible Cre-C; Cre-230C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-FHY1

Use

Cre/Lox

Expression

Mammalian

Mutation

None

Promoter

CAGG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT367

Plasmid

#233673

Purpose

Red light-inducible Cre-N; Cre-N229-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N229-FHY1

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT336

Plasmid

#233662

Purpose

AND Gate Reporter to identify Cre and Flp recombination; FRT-STOP-FRT-loxP-STOP-loxP-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-loxP-STOP-loxP-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS794

Plasmid

#238207

Purpose

Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Mutation

A insertion at K85 codon

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3220

Plasmid

#234099

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3237

Plasmid

#234091

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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