We narrowed to 26,138 results for: Vit

1. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

   Plasmid

   #63059

   Purpose

   Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   PKIalpha (Pkia Synthetic, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP469 pEF1a-mCherry-EMPTY-gRNA

   Plasmid

   #100958

   Purpose

   mCherry driven by EF1a, empty gRNA scaffold driven by U6

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   gRNA empty backbone with RFP
   

   Use

   CRISPR

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-MTOR-I2500F

   Plasmid

   #69014

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-I2500F (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Isoleucine 2500 to Phenylalanine

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIA3-Pansio

   Plasmid

   #214445

   Purpose

   Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.23 WT 2014 EBOV + Mucin-Like Domain

   Plasmid

   #85794

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Pardis Sabeti, Jeremy Luban

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. non-targeting control gRNA (BRDN0001145694)

   Plasmid

   #80191

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLF2-GFP-3xFLAG

   Plasmid

   #230986

   Purpose

   Expresses GFP tagged MLF2 in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

   Insert

   MLF2 (MLF2 Human)
   

   Use

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-Tev-Flag-Ube2v2

   Plasmid

   #110070

   Purpose

   Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

   Depositor

   Yimon Aye

   Article

   Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

   Insert

   Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
   

   Use

   Tags

   Halo-TEV-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

   Plasmid

   #100846

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag-hNF2

    Plasmid

    #107150

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIA10.5-Clover

    Plasmid

    #214449

    Purpose

    BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    mClover
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPHK1 gRNA (BRDN0001147454)

    Plasmid

    #76813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. S1PR1-DuET

    Plasmid

    #213374

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR1 (S1PR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER13 (miR-LUP)

    Plasmid

    #46936

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 TO

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 608 pSG5L HA RB del ex4

    Plasmid

    #10730

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del exon4 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of exon4

    Promoter

    Available since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

18. 498 pSG5L HA RB del663 (NAAIRS)

    Plasmid

    #10728

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del663 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 664 to 669 were replaced by NAAIRS, a fl…

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGW-Dronpa-Cav1.2

    Plasmid

    #182333

    Purpose

    L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Cav1.2-Dronpa (CACNA1C Rabbit)
    

    Use

    Tags

    Full-length rabbit Cav1.2.

    Expression

    Mammalian

    Mutation

    T1066Y, Q1070M

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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