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pMC_cherry donor_Polb target (pE038)

Plasmid

#149349

Purpose

Replace donor vector for cherry insertion using the human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAF302

Plasmid

#137752

Purpose

Intracellular control plasmid for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

hupA-linker-hibitopt

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAP233

Plasmid

#137753

Purpose

Extracellular control for HiBiTopt experiments

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Bacteriol. 2020 Aug 31. pii: JB.00771-

Insert

srtB-linker-hibitopt

Use

Luciferase

Tags

HiBiTopt

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymVenus-T2A-mTurquoise2

Plasmid

#118443

Purpose

Produces equimolar expression of yeast codon-optimized mVenus and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yotagRFPT-T2A-mTurquoise2

Plasmid

#118449

Purpose

Produces equimolar expression of yeast codon-optimized tagRFPT and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yotagRFPT-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0012

Plasmid

#117584

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Mutation

D1135E

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pVH011

Plasmid

#80395

Purpose

Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Insert

Bicistronic Scaffold-3xFLAG-SD-mCherry

Use

Synthetic Biology

Tags

3xFLAG tag on scaffold protein for Western

Expression

Bacterial

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRAJ12f

Plasmid

#39248

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no terminator for the transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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