We narrowed to 9,614 results for: Coli
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Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB
Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
ExpressionBacterialAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorAvailable SinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only