We narrowed to 16,583 results for: EMB

1. myc-BioID-Rab7

   Plasmid

   #121049

   Purpose

   Expresses myc tagged BioID and human Rab7a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   Rab7A
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF(UG) hSyn SYP-mRuby3

   Plasmid

   #179727

   Purpose

   Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYP (Syp Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Pert Heavy chain-SpyTag003

   Plasmid

   #216309

   Purpose

   Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS pEGFP-N3 (1299)

   Plasmid

   #62043

   Purpose

   mammalian expression of NLS-EGFP

   Depositor

   Eric Schirmer

   Article

   Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

   Insert

   SV40 NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-Skp2

   Plasmid

   #19946

   Depositor

   Yue Xiong

   Article

   Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

   Insert

   S-phase kinase-associated protein 2 (p45) (SKP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT PUR mGSK3AWT

   Plasmid

   #35159

   Depositor

   Jim Woodgett

   Article

   Doble et al Dev Cell. 2007 Jun;12(6):957-71.

   Insert

   GSK-3α (Gsk3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. AICSDP-078:G3BP1-mEGFP

   Plasmid

   #193920

   Purpose

   Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

   Depositor

   Allen Institute for Cell Science

   Insert

   G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-alpha4 wt

   Plasmid

   #80016

   Purpose

   Mammalian expression of human integrin alpha4

   Depositor

   Chinten James Lim

   Article

   Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

   Insert

   integrin alpha4 (ITGA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLVX-Tight-Puro_ST18

   Plasmid

   #64844

   Purpose

   A lentiviral vector for the expression of mouse ST18 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   ST18 (St18 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL_dstβL

    Plasmid

    #73805

    Purpose

    Plasmid for selection for insertion and homodimerization

    Depositor

    Sarel Fleishman

    Article

    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

    Inserts

    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    

    Use

    Tags

    Part Of the dstbl chiemra, Part of the dsTbL chim…

    Expression

    Bacterial

    Mutation

    Promoter

    NaN and ToxR promotor

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. STAGR_gRNAScaffold_mU6

    Plasmid

    #102844

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pHR-CMV-TetO2_mRuby2

    Plasmid

    #113894

    Purpose

    Mammalian (inducible) expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Ruby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pdgfra-T2A-H2B-eGFP donor

    Plasmid

    #113120

    Purpose

    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

    Plasmid

    #86417

    Purpose

    luminal split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-mCherry-SCS2TM-GFP11
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPyPGK-EGFP-TM-IRES-Pac

    Plasmid

    #183604

    Purpose

    Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    Human PDGFRB transmembrane domain and Mouse IgGK …

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Pgk1

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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