We narrowed to 26,307 results for: Vit

1. pMIA10.53-Clover

   Plasmid

   #214450

   Purpose

   BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   mClover
   

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.PHF19

   Plasmid

   #125168

   Purpose

   Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF19 (PHF19 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABE8.13-m

   Plasmid

   #136296

   Purpose

   expresses ABE8.13-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.13-m
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-HA-GOLPH3

   Plasmid

   #21687

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. VN-Sph1

   Plasmid

   #89755

   Purpose

   expresses human Synphilin fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

   Insert

   SNCAIP (SNCAIP Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-FLAG-APEX2-LGALS3

   Plasmid

   #175756

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MRGPRX1-DuET

    Plasmid

    #213344

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX1 (MRGPRX1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P CISD1_2

    Plasmid

    #160774

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST_FLAG-HUWE1_delta169-189

    Plasmid

    #187136

    Purpose

    mammalian cell expression of FLAG-HUWE1 delta169-189

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    delta169-189

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 bovine GRK5

    Plasmid

    #14690

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK5 (GRK5 Bovine)
    

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

14. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TNIK gRNA (BRDN0001146045)

    Plasmid

    #75849

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINDUCER13 (miR-LUP)

    Plasmid

    #46936

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

    Plasmid

    #232244

    Purpose

    Mammalian expression of N581K RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    N581K RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    N581K

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

    Plasmid

    #232240

    Purpose

    Mammalian expression of REX1[degron]-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    REX1 (aa 70-85)

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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