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pcDNA3.1-FGFR2c-V5/HIS

Plasmid

#201107

Purpose

expression of human FGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFR_HCV_xb

Plasmid

#11510

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Expression

Mammalian

Promoter

hU6 and EFS

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA.3.1.ADRB2-NP

Plasmid

#134376

Purpose

Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

ADRB2-NP (ADRB2 Human)

Tags

Flag, natural peptide of nanoluciferase, and sign…

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE4

Plasmid

#197148

Purpose

Expresses ApoE4 in Mammalian cell

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE4 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pR-mKate2

Plasmid

#228610

Purpose

Mammalian expression of mKate2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-kanMX6

Plasmid

#20754

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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EFS-GFP

Plasmid

#110834

Purpose

positive control for GFP expression

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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