Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 19,353 results for: MUT

Showing: 8541 - 8560 of 19353 results

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA + S580A POLI

Plasmid

#131241

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert Pol iota se…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA targeting human SLC12A5 gene stop codon

Plasmid

#158577

Purpose

For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

Depositor

Rudolf Jaenisch

Article

Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

Insert

sgRNA targeting human SLC12A5 gene stop codon

Use

CRISPR

Tags

Cas9 and GFP

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-T92Q-Fc(IgA1)

Plasmid

#145162

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-NT-G46D

Plasmid

#134588

Purpose

Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G154D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-FL-G84D

Plasmid

#134577

Purpose

Express human DDX43 mutant (full-length with G84D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-G84D (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G84D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta95-114

Plasmid

#124925

Purpose

For expression of a.a.95-114 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta116-127

Plasmid

#124926

Purpose

For expression of a.a.116-127 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-R240C

Plasmid

#116680

Purpose

Lentiviral expression of SIRPB1 R240C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

R240C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-W183*

Plasmid

#116681

Purpose

Lentiviral expression of SIRPB1 W183*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

W183*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMAD4-K436N

Plasmid

#116682

Purpose

Lentiviral expression of SMAD4 K436N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Mutation

K436N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK19-D89N

Plasmid

#116688

Purpose

Lentiviral expression of STK19 D89N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK19 (STK19 Human)

Use

Lentiviral

Mutation

D89N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-D198N

Plasmid

#116676

Purpose

Lentiviral expression of SIRPB1 D198N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

D198N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-E170K

Plasmid

#116677

Purpose

Lentiviral expression of SIRPB1 E170K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

E170K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-G290E

Plasmid

#116678

Purpose

Lentiviral expression of SIRPB1 G290E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

G290E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SIRPB1-R124Q

Plasmid

#116679

Purpose

Lentiviral expression of SIRPB1 R124Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

R124Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1-D894N

Plasmid

#116611

Purpose

Lentiviral expression of PTCH1 D894N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

D894N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1-G1212D

Plasmid

#116612

Purpose

Lentiviral expression of PTCH1 G1212D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

G1212D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1-R1308Kfs*64

Plasmid

#116613

Purpose

Lentiviral expression of PTCH1 R1308Kfs*64

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

R1308Kfs*64

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTCH1-S1203Rfs*52

Plasmid

#116614

Purpose

Lentiviral expression of PTCH1 S1203Rfs*52

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Mutation

S1203Rfs*52

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 8541 - 8560 of 19353 results

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