We narrowed to 14,443 results for: NTS

1. pDONR221 NLRP1 delta787-843

   Plasmid

   #166821

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   _787-843

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 Ub-NLRP1 S1213 P1214R

   Plasmid

   #166830

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Mutation

   P1214R

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 NLRP1 T755N

   Plasmid

   #166820

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   T755N

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 NLRP1 R726W

   Plasmid

   #166819

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   R726W

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

   Plasmid

   #164132

   Purpose

   Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   ciCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPK-352

   Plasmid

   #157922

   Purpose

   pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   tPcyA
   IRES
   HO1
   FD
   FNR
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   Mitochondrial Targeting Sequence, Mitochondrial T…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRC/CMV-TYK2VF/PA-VSV

   Plasmid

   #139351

   Purpose

   Expression of the double mutant of TYK2-V678F-P1104A

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-V678F-P1104A

   Promoter

   CMV

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BtGRK2[D110A K220R]-mVenus

   Plasmid

   #137779

   Purpose

   Visualization of GRK2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Bovine GRK2[D110A K220R] (GRK2 Bovine)
   

   Expression

   Mammalian

   Mutation

   D110A K220R

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126758

   Purpose

   Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126759

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126753

    Purpose

    Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126754

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126756

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HsGRK6[L66A E514A]-sYFP2

    Plasmid

    #137781

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6[L66A E514A] (GRK6 Human)
    

    Expression

    Mammalian

    Mutation

    L66A E514A

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

    Plasmid

    #136063

    Purpose

    Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Y84C/C121S/A139C

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-HLA-B15-VN

    Plasmid

    #135514

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-HLA-B57-VN

    Plasmid

    #135515

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-HLA-B8-VN

    Plasmid

    #135511

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*08:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*08:01 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PV259

    Plasmid

    #132340

    Purpose

    CasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    CasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PV503

    Plasmid

    #132342

    Purpose

    CasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    CasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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