2,294 results

1. sh-SRF

   Plasmid

   #100797

   Purpose

   expression of shRNA targeting SRF

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

   Insert

   rat SRF
   

   Use

   RNAi

   Promoter

   H1

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CC10-rtTA

   Plasmid

   #20179

   Depositor

   Jeffrey Whitsett

   Article

   Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-

   Insert

   rcc10 promoter
   

   Expression

   Mammalian

   Available Since

   March 12, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pSynapsin_NLS_GFP_P2A_SNAP_NR1

   Plasmid

   #170458

   Purpose

   Expresses SNAP tagged NR1 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AU1-mTOR-L1460P

   Plasmid

   #19995

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-L1460P (Mtor Rat)
   

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Leucine 1460 to Proline

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. EMSV-myogenin (puro)

   Plasmid

   #14712

   Depositor

   Michael Rudnicki

   Article

   Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

   Insert

   myogenin (Myog Rat)
   

   Expression

   Mammalian

   Available Since

   April 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLC3sHA

   Plasmid

   #52424

   Purpose

   mammalian expression of HA tagged rat CLC3 short transcript

   Depositor

   Steven Weinman

   Article

   Wang et al Gastroenterology. 2004 Apr;126(4):1157-6

   Insert

   CLC3 (Clcn3 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-sACt-mCherry-NES

   Plasmid

   #138215

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NES (Adcy10 Rat)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKC zeta WT

   Plasmid

   #21248

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC zeta (Prkcz Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. AllCB shRNA-1

    Plasmid

    #160065

    Purpose

    Knock Down all Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

    Plasmid

    #165445

    Purpose

    Expresses ACX, rPB(8kD) in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rPB(8kD)
    

    Expression

    Mammalian

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. α1GA-HE3-pcDNA3

    Plasmid

    #45812

    Depositor

    Edward Perez-Reyes

    Insert

    α1Ga (Cacna1g Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBa-FRB-3myc-KIF1A tail 391-1698

    Plasmid

    #64286

    Purpose

    KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    KIF1A (Kif1a, Kif1a Rat, Mouse)
    

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    aa 391-1698, 5'end mostly matches rat, 3…

    Promoter

    chicken Beta Actin

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. G protein alpha-s(alpha3beta5)

    Plasmid

    #55799

    Purpose

    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…

    Promoter

    CMV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-tdTomato-P2A-paCaMKII(T286A)

    Plasmid

    #165432

    Purpose

    Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII(T286A)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    T286A: Autophosphorylation deficient mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SnFR-gamma8

    Plasmid

    #165496

    Purpose

    Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pMK277 (mClover-NeoR)

    Plasmid

    #72793

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Neo
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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