We narrowed to 2,323 results for: PUC19

1. qTAG-N-Puro-mNeon

   Plasmid

   #207687

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-N-Puro-3xFLAG

   Plasmid

   #207695

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. qTAG-C-sTagRFP-Puro

   Plasmid

   #207723

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. AICSDP-28:TUBA1B-mTagRFP-T

   Plasmid

   #101785

   Purpose

   Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Insert

   TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFPT-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. qTAG-C-3xHA-Blast

   Plasmid

   #207718

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. qTAG-N-Puro-mScarlet

   Plasmid

   #207689

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-N-Blast-V5

   Plasmid

   #207686

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. qTAG-N-Blast-miniTurbo

   Plasmid

   #207681

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. qTAG-C-3xFLAG-Puro

   Plasmid

   #207728

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-C-mNeon-Puro

    Plasmid

    #207720

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-N-Zeo-3xFLAG

    Plasmid

    #207706

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-N-Blast-3xHA

    Plasmid

    #207685

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAT15068-evoCBE

    Plasmid

    #163013

    Purpose

    plasmid expressing evoCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMIN-Ri

    Plasmid

    #197784

    Purpose

    Level 2 destination vector: pMIN-Ri backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAT15489_dABE8e

    Plasmid

    #174127

    Purpose

    plasmid expressing ABE8e with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEED-mScarlet3:ALFA

    Plasmid

    #219641

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR562

    Plasmid

    #207886

    Purpose

    Creation of Level 0 entry clones

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    amilCP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-C-3xFLAG-Zeo

    Plasmid

    #207739

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. qTAG-C-V5-Blast

    Plasmid

    #207719

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pSEED-mCherry

    Plasmid

    #219657

    Purpose

    Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. qTAG-N-Blast-dTAG

    Plasmid

    #207683

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. qTAG-N-Puro-miniTurbo

    Plasmid

    #207692

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. SS9_RNA

    Plasmid

    #71656

    Purpose

    SS9-targeting gRNA for co-transformation with pSS9

    Depositor

    Ryan Gill

    Article

    Bassalo et al ACS Synth Biol. 2016 Apr 22.

    Insert

    SS9 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-N-Puro-ultraID

    Plasmid

    #207693

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

27. qTAG-C-mNeon-Blast

    Plasmid

    #207709

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. qTAG-C-miniTurbo-Blast

    Plasmid

    #207714

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pSEED-Dendra2

    Plasmid

    #219645

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Dendra2 SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAT15482_ABE8e

    Plasmid

    #174120

    Purpose

    Expresses ABE8e with an EF1a promoter

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge