Addgene

We narrowed to 8,731 results for: control

Showing: 1261 - 1290 of 8731 results

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry

Plasmid

#207668

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP)

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

MCP-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAG1160 mCherry-FIREtag

Plasmid

#205164

Purpose

Expresses mCherry -FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP

Plasmid

#179636

Purpose

TEVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-N144

Plasmid

#66810

Purpose

2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

Reverse tetracycline-controlled transactivator

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-LEXY (pDN138)

Plasmid

#72657

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

NLS and mCherry-LEXY

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

MBP and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRPLC

Plasmid

#122261

Purpose

pBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Nov 16;7(11):2627-2

Insert

pBRBAD, pBRlasI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp Phy-mCherry-CAAX

Plasmid

#50839

Purpose

Optogenetic PI3K activation system; membrane component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PhyB 1-908

Use

Lentiviral and Synthetic Biology

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNDL-54

Plasmid

#161715

Purpose

In vitro transcription construct for production of membrane acvr1b-Cry2 mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

Myr-acvr1b-Cry2 (acvr1ba Zebrafish)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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p006-GFP-strong

Plasmid

#108313

Purpose

GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFAB4026

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PKC alpha WT

Plasmid

#21232

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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ILID-CAAX

Plasmid

#85680

Purpose

Expression of untagged ILID CAAX

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

ILID-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMag(3x)-iRFP

Plasmid

#67305

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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