We narrowed to 2,493 results for: ERK

1. pGAL4-DBD-HEY2-Adel-IDR

   Plasmid

   #145234

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HEY2-Adel-IDR (HEY2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGAL4-DBD-BHLHE41-Adel-IDR

   Plasmid

   #145228

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   BHLHE41-Adel-IDR (BHLHE41 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEHISSSO6206

   Plasmid

   #97065

   Purpose

   Expressing Sulfolobus solfataricus orf SSO6206 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO6206
   

   Use

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBabe-Puro-MEK-DD

   Plasmid

   #15268

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   Map2k1 (Mapk1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S218D S222D

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

   Plasmid

   #128605

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   24xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDECKO_GFP

   Plasmid

   #141210

   Purpose

   empty GFP vector for cloning sgRNA or pgRNAs

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLHCX-ATF4 mScarlet NLS

   Plasmid

   #115970

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-MCS-MED1long-IDR-YFP-NLS

    Plasmid

    #145276

    Purpose

    Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MED1-IDR long (MED1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid

    #89463

    Purpose

    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Use

    Tags

    E. coli DHFRdd destabilization domain and mCherry…

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC1-V16-Luc

    Plasmid

    #122034

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145270

    Purpose

    Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(53Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145269

    Purpose

    Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(38Q)-IDR (TBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p2K7bsdUBI-mCherry-STIM1

    Plasmid

    #114178

    Purpose

    Lentiviral vector for expression of mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Budding Yeast, Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

    Plasmid

    #65446

    Purpose

    Expression of ephrinB1 and RFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    ephrin B1 (Efnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pENTR1a-mCherry-STIM1

    Plasmid

    #114176

    Purpose

    Gateway entry clone containing mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Gateway entry clone

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEAHISPApqsE

    Plasmid

    #97019

    Purpose

    Expressing Pseudomonas aeruginosa pqsE protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Quinolone signal response protein
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEAHISMRSA2676

    Plasmid

    #96970

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 2676

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Sar2676
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEAHISMRSAMVAK

    Plasmid

    #97000

    Purpose

    expression of methicillin-resistant Staphylococcus aureus MVAK protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Mevalonate kinase
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEAHISPAFabH

    Plasmid

    #97018

    Purpose

    Expressing Pseudomonas aeruginosa FabH protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    3-Oxoacyl-[acyl-carrier-protein] synthase III
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAPK15 gRNA (BRDN0001145787)

    Plasmid

    #75618

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK15

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK15 (MAPK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAPK15 gRNA (BRDN0001148491)

    Plasmid

    #75619

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK15

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK15 (MAPK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAPK15 gRNA (BRDN0001162376)

    Plasmid

    #75620

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK15

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK15 (MAPK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAPK15 gRNA (BRDN0001162374)

    Plasmid

    #75621

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK15

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK15 (MAPK15 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAPK7 gRNA (BRDN0001148313)

    Plasmid

    #76894

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK7

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK7 (MAPK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MAPK7 gRNA (BRDN0001145201)

    Plasmid

    #76895

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK7

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK7 (MAPK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MAPK7 gRNA (BRDN0001145053)

    Plasmid

    #76896

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK7

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK7 (MAPK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145272

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(-15A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat deleted

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEAHISSSO2462

    Plasmid

    #97064

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2462 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2462
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEAHISSSO2452

    Plasmid

    #97059

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2452 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2452
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEAHISPSVORF126

    Plasmid

    #97021

    Purpose

    Expressing Pyrobaculum spherical virus orf126 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 126
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEAHISPA5006

    Plasmid

    #97016

    Purpose

    Expressing Pseudomonas aeruginosa orf PA5006 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    P aeruginosa orf PA5006
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDEHISSIRV119

    Plasmid

    #97054

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 119
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEHISSIRV55

    Plasmid

    #97055

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 55
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEANHISECArdB

    Plasmid

    #96967

    Purpose

    Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    E. coli ArdB
    

    Use

    Tags

    N-ter TEV cleavable 6His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge