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pJK392

Plasmid

#70236

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Expression

Bacterial

Mutation

His40Asn

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PYBS375

Plasmid

#18887

Depositor

Elaine Elion

Article

Choi et al Cell. 1994 Aug 12. 78(3):499-512.

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Mutation

Lex-Ste11C (amino-terminal 413 amino acids)

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pICH-roGFP2-synORP1

Plasmid

#191680

Purpose

Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

rredox(roGFP2-synORP1

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai2

Plasmid

#16369

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai2 (ORAI2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pMET17p-CRU313

Plasmid

#131163

Purpose

For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

CRU

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFA CUP1p-NuiHA kanMX

Plasmid

#131169

Purpose

PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pR-spondin 1

Plasmid

#128844

Purpose

bacterial expression of pR-spondin 1

Depositor

Marc de la Roche

Article

Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

Insert

R-spondin 1 (RSPO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-FLAG-dN6-His

Plasmid

#22143

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

Flag-clpXdeltaNLinkedHexamer-His6

Tags

Flag, His6, and L20 Linkers

Expression

Bacterial

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJUMP25-1A(sfGFP)

Plasmid

#126972

Purpose

Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP47-2A(sfGFP)

Plasmid

#126991

Purpose

Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

Plasmid

#148113

Purpose

Mammalian Expression of HshnRNPA2-B1

Depositor

Elisa Izaurralde

Insert

HshnRNPA2-B1 (HNRNPA2B1 Human)

Expression

Mammalian

Mutation

splicing variant of hnRNPA2 variant B1. Exon 6 an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RPA40

Plasmid

#17658

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA40 (POLR1C Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pJBL3752

Plasmid

#128809

Purpose

Fluoride riboswitch-regulated sfGFP

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-hACE2-cMYC-Flag

Plasmid

#158957

Purpose

Used for expression of human ACE2 with AAV packaging

Depositor

Akiko Iwasaki

Insert

Human ACE2 (ACE2 Human)

Use

AAV

Tags

Flag and MYC

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP21-1A(sfGFP)

Plasmid

#126968

Purpose

Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT2

Plasmid

#122140

Purpose

For making cRNA appropriate for microinjection

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Type

Empty backbone

Use

For in vitro transcription

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJG/ Inducible ALPHA MHC

Plasmid

#55792

Purpose

transgene expression driven by inducible ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

Type

Empty backbone

Expression

Mammalian

Promoter

Inducible ALPHA MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Syt1 C2AB (96-421)

Plasmid

#170643

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-RPA16

Plasmid

#17657

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA16 (POLR1D Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only