Addgene

We narrowed to 2,333 results for: Efs

Showing: 841 - 860 of 2333 results

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pScCas9

Plasmid

#117700

Purpose

Mammalian expression vector encoding WT ScCas9

Depositor

Joseph Jacobson

Article

Chatterjee et al Sci Adv. 2018 Oct 24;4(10):eaau0766. doi

Insert

Streptococcus canis Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTs

Plasmid

#31173

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Ts

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSLQ11440

Plasmid

#183203

Purpose

pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

hyperdCas12 base editor

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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iDuet101a

Plasmid

#17629

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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sg_shuttle_RFP657

Plasmid

#134968

Purpose

Lentiviral vector for sgRNA expression

Depositor

Beat Bornhauser

Article

Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6-sgRNA; EFS-RFP657

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKER-mKOk

Plasmid

#85975

Purpose

Expresses mKOk from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mKOk

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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XX01CAPN1A-c001

Plasmid

#175491

Purpose

Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN1 / CAPNS1 (CAPN1 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKER-mRuby2

Plasmid

#85977

Purpose

Expresses mRuby2 from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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TALE8

Plasmid

#35423

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE8

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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XX01CAPN2A-c001

Plasmid

#175492

Purpose

Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN2 / CAPNS1 (CAPN2 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tNub

Plasmid

#154039

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Nub

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tPbt

Plasmid

#154043

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Pbt

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKER-mCardinal

Plasmid

#85976

Purpose

Expresses mCardinal from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mCardinal

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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TALE5

Plasmid

#35420

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE5

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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TALE10

Plasmid

#35425

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE10

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBC2101-reci

Plasmid

#117170

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 with SV40 NLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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TALE12

Plasmid

#35427

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE12

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TALE11

Plasmid

#35426

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE11

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge