We narrowed to 1,413 results for: Flp

1. pcDNA5/FRT/TO_eGFP-pScaE

   Plasmid

   #228826

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaE
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 49-494

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_eGFP-pScaD

   Plasmid

   #228825

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaD
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 30-700

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO_eGFP-pScaC

   Plasmid

   #228824

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_eGFP-pScaA

   Plasmid

   #228823

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaA
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   aa 25-1216

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5 FRT-TO-EGFP-UPF1-R615A

   Plasmid

   #136001

   Purpose

   DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R615A

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 FRT-TO-EGFP-UPF1-DEAA

   Plasmid

   #136002

   Purpose

   DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   DE647AA

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSYN1-fDIO-tTA

   Plasmid

   #166597

   Purpose

   Encodes Flp-dependent tTA

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   tTA
   

   Use

   AAV

   Promoter

   human synapsin 1

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pADH100

   Plasmid

   #90980

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ConVERGD-eGFP-W3SL

    Plasmid

    #218742

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-ConVERGD-eGFP-WPRE

    Plasmid

    #218749

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-ConVERGD-eGFP-W3SL

    Plasmid

    #218748

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-Ef1a-ConVERGD-eGFP-WPRE

    Plasmid

    #218745

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RNA-Protein Landing Pad

    Plasmid

    #226162

    Purpose

    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSYN1-fDIO-rtTA

    Plasmid

    #166598

    Purpose

    Encodes Flp-dependent rtTA

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    rtTA
    

    Use

    AAV

    Promoter

    human synapsin 1

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCL1161

    Plasmid

    #217964

    Purpose

    For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3.1 (H3C1 Human)
    

    Tags

    eGFP, APEX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ConVERGD-ConFonvCon-eGFP-W3SL

    Plasmid

    #218752

    Purpose

    Provides AND intersectional (Cre+Flp+vCre) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-ConVERGD-ConFoff-eGFP-W3SL

    Plasmid

    #218751

    Purpose

    Provides NOT intersectional (Cre NOT Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-ConVERGD-TVA(mChr)-W3SL

    Plasmid

    #218754

    Purpose

    Provides AND intersectional (Cre+Flp) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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