Addgene

We narrowed to 7,339 results for: Lif

Showing: 841 - 860 of 7339 results

Results per page:

Sort results by:

pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc V5 dDispatched WT HA

Plasmid

#126405

Purpose

Expression of V5 dDispatched WT HA in Drosophila cells lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSYN1-fDIO-tTA

Plasmid

#166597

Purpose

Encodes Flp-dependent tTA

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

tTA

Use

AAV

Tags

Expression

Mutation

Promoter

human synapsin 1

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pCMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Paxillin WT

Plasmid

#186152

Purpose

For expressing paxillin variants in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CaNAR2

Plasmid

#64728

Purpose

FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Use

Tags

Cerulean3 and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
N1-G-Ca-FLITS

Plasmid

#191465

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS:TVA-mCherry

Plasmid

#187823

Purpose

Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.

Depositor

Rainer Friedrich

Article

Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi:

Insert

TVA-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

5xUAS

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
epB_UbC_CymRV5-nls-Halox2_DEx4

Plasmid

#119907

Purpose

ePiggyBac mammalian expression plasmid for CymR-Halo fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-Halox2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-TurboID-Mfn1

Plasmid

#195155

Purpose

mammalian expression of TurboID tagged Mfn1

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

TurboID

Use

Tags

Mfn1

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

CMV

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-Syt::Halo7

Plasmid

#87648

Purpose

For creating drosophila transgenics expressing UAS-Syt::Halo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::Halo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK1024 (pDS-Def1)

Plasmid

#183125

Purpose

Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

Fragment of Def1 gene

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-TurboID-Mfn1E209A

Plasmid

#195156

Purpose

mammalian expression of TurboID tagged Mfn1E209A

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

TurboID

Use

Tags

Mfn1-E209A

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

CMV

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_083 Cas12i1

Plasmid

#171670

Purpose

For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-mRuby3

Plasmid

#179730

Purpose

Mammalian expression of mRuby3 from the EF1 promoter

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-mTagBFP2-TEV-Strep-His

Plasmid

#210824

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mTagBFP2

Use

Tags

TwinStrep, 10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge