We narrowed to 1,130 results for: Tef
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Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-GFP-SPOP (R354H)
Plasmid#197010PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-GFP-SPOP (R221C)
Plasmid#197009PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-SPOP-myc (R121Q)
Plasmid#197007PurposeMammalian inducible expression vector for myc-tagged SPOPDepositorAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-SPOP-myc (R354H)
Plasmid#197006PurposeMammalian inducible expression vector for myc-tagged SPOPDepositorAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-SPOP-myc (R221C)
Plasmid#197005PurposeMammalian inducible expression vector for myc-tagged SPOPDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-SPOP-myc (BACK)
Plasmid#197021PurposeMammalian inducible expression vector for myc-tagged SPOPDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T-
Plasmid#161503PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T-
Plasmid#161497PurposeNon activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0649
Plasmid#129010PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0650
Plasmid#129011PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0648
Plasmid#129009PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0651
Plasmid#129012PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only