We narrowed to 13,942 results for: cas9

1. pBbdCas9S_PresTL-sgRNAflaB

   Plasmid

   #149645

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, PresTL

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbdCas9S_P0026-sgRNAflaB

   Plasmid

   #149646

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0026

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S_P0826S-sgRNAflaB

   Plasmid

   #149647

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0826S

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S_Psyn-sgRNAftsI

   Plasmid

   #149649

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9S_P0526-sgRNAftsI

   Plasmid

   #149650

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0526

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSM-dCas9::Ap

   Plasmid

   #154307

   Purpose

   Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9n-Nanog-L

   Plasmid

   #122302

   Purpose

   Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9n-Nanog-R

   Plasmid

   #122303

   Purpose

   Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic, Mouse)
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-L3-Cas9n-L2

   Plasmid

   #141039

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9n
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p415:iCas9

    Plasmid

    #138266

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Cas9-D10A, H840A

    Promoter

    GalL

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid

    #136657

    Purpose

    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid

    #136654

    Purpose

    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid

    #127908

    Purpose

    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Human 5' HP1beta
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA2963_eSpCas9-PGKHygdtkCh

    Plasmid

    #124205

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJUMP18-dCas9_O

    Plasmid

    #127028

    Purpose

    Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part dCas9_O
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-J23113

    Plasmid

    #119701

    Purpose

    Expression of Cas9 with a weak promoter

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSD_SpCas9MT3-FKBP_SL

    Plasmid

    #107302

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. St1Cas9_LMD-9_sgRNA_pUC19

    Plasmid

    #110627

    Purpose

    U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Insert

    Empty St1Cas9 LMD-9 sgRNA backbone
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAtCas9-D10A_1

    Plasmid

    #91159

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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