We narrowed to 14,467 results for: cas9 genes

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pBLO43.3_human ProCas9Flavi

Plasmid

#121629

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTaCas9-H840A_5

Plasmid

#91172

Purpose

Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_6

Plasmid

#91171

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_3

Plasmid

#91161

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pD10AH840AhCas9 (GB1041)

Plasmid

#68207

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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CYLBL_Neo_TREdCas9-KRAB

Plasmid

#251231

Purpose

TRE-dCas9-KRAB knock-in using TALEN at CLYBL locus (derived from Plasmid #220439)

Depositor

Gary Hon

Insert

TRE-dCas9 KRAB

Use

CRISPR and TALEN

Tags

KRAB

Expression

Mammalian

Mutation

D10A and H840A

Promoter

TRE

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT)

Plasmid

#251549

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_WT_GFP (ABE8e_V28C_nCas9_WT)

Plasmid

#251548

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY)

Plasmid

#251544

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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dCas9-KYP (M6)

Plasmid

#239480

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-dCas9-2XNLS-XTEN16-BFP-KYP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-ryt

Plasmid

#246022

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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EML8357_pA3A-CBECas9

Plasmid

#234352

Purpose

A base editor derived by egg-cell specific promoter

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9

Plasmid

#248566

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV, TetO2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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