We narrowed to 1,266 results for: grna cloning vector

1. memb-mNeongreen

   Plasmid

   #241175

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp2-SmuCas9_AAV

   Plasmid

   #192144

   Purpose

   Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2-SmuCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231362

   Purpose

   minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SA-mCherry-pA

   Plasmid

   #241212

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mCherry

   Depositor

   Junsu Kang

   Insert

   mCherry
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SA-EGFP-pA

   Plasmid

   #241211

   Purpose

   Middle entry vector for mini-Golden subcloning platform; eGFP

   Depositor

   Junsu Kang

   Insert

   eGFP
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SA-TCGC-mNeongreen

   Plasmid

   #241203

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SA-memb-mNeongreen

   Plasmid

   #241207

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMid-TCGC-mNeongreen

   Plasmid

   #241171

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lifeact-mNeongreen-pA

    Plasmid

    #241202

    Purpose

    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

    Depositor

    Junsu Kang

    Insert

    lifeact-mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. linker-P2A-mNeongreen-ORF1

    Plasmid

    #241176

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. linker-P2A-mNeongreen-ORF2

    Plasmid

    #241177

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. linker-P2A-mNeongreen-ORF3

    Plasmid

    #241178

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FRT-acrymCherry-FRT

    Plasmid

    #241179

    Purpose

    Middle entry vector for mini-Golden subcloning platform; acry-mCherry

    Depositor

    Junsu Kang

    Insert

    acry-mCherry
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-multi-EBFP2-NLS (pRW1447)

    Plasmid

    #216219

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SA-linker-P2A-mNeongreen-ORF1

    Plasmid

    #241208

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SA-linker-P2A-mNeongreen-ORF2

    Plasmid

    #241209

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SA-linker-P2A-mNeongreen-ORF3

    Plasmid

    #241210

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    TCGC linker

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge