Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,273 results for: grna cloning vector

Clear keywords
Showing: 841 - 860 of 1273 results

  1. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

    Enlarge

  5. SA-mCherry-pA

    Plasmid
    #241212
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mCherry
    Depositor
    Junsu Kang
    Insert
    mCherry
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241212-plasmid-map-sequence-id-484620

    Enlarge

  6. SA-EGFP-pA

    Plasmid
    #241211
    Purpose
    Middle entry vector for mini-Golden subcloning platform; eGFP
    Depositor
    Junsu Kang
    Insert
    eGFP
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241211-plasmid-map-sequence-id-482022

    Enlarge

  7. SA-TCGC-mNeongreen

    Plasmid
    #241203
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241203-plasmid-map-sequence-id-484622

    Enlarge

  8. SA-memb-mNeongreen

    Plasmid
    #241207
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241207-plasmid-map-sequence-id-482175

    Enlarge

  9. pMid-TCGC-mNeongreen

    Plasmid
    #241171
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241171-plasmid-map-sequence-id-482081

    Enlarge

  12. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

    Enlarge

  13. lifeact-mNeongreen-pA

    Plasmid
    #241202
    Purpose
    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen
    Depositor
    Junsu Kang
    Insert
    lifeact-mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241202-plasmid-map-sequence-id-482031

    Enlarge

  14. linker-P2A-mNeongreen-ORF1

    Plasmid
    #241176
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241176-plasmid-map-sequence-id-482017

    Enlarge

  15. linker-P2A-mNeongreen-ORF2

    Plasmid
    #241177
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241177-plasmid-map-sequence-id-482016

    Enlarge

  16. linker-P2A-mNeongreen-ORF3

    Plasmid
    #241178
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241178-plasmid-map-sequence-id-482014

    Enlarge

  17. FRT-acrymCherry-FRT

    Plasmid
    #241179
    Purpose
    Middle entry vector for mini-Golden subcloning platform; acry-mCherry
    Depositor
    Junsu Kang
    Insert
    acry-mCherry
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241179-plasmid-map-sequence-id-482012

    Enlarge

  20. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

    Enlarge

Showing: 841 - 860 of 1273 results