We narrowed to 1,270 results for: grna cloning vector

1. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-multi-Zeo (pRW1445)

   Plasmid

   #216218

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCS_BsmBI

   Plasmid

   #241170

   Purpose

   Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI

   Depositor

   Junsu Kang

   Insert

   MCS; BsmBI
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. memb-mNeongreen

   Plasmid

   #241175

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nsp2-SmuCas9_AAV

   Plasmid

   #192144

   Purpose

   Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2-SmuCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231362

   Purpose

   minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231363

   Purpose

   Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. SA-mCherry-pA

    Plasmid

    #241212

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mCherry

    Depositor

    Junsu Kang

    Insert

    mCherry
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. SA-EGFP-pA

    Plasmid

    #241211

    Purpose

    Middle entry vector for mini-Golden subcloning platform; eGFP

    Depositor

    Junsu Kang

    Insert

    eGFP
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SA-TCGC-mNeongreen

    Plasmid

    #241203

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SA-memb-mNeongreen

    Plasmid

    #241207

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMid-TCGC-mNeongreen

    Plasmid

    #241171

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. lifeact-mNeongreen-pA

    Plasmid

    #241202

    Purpose

    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

    Depositor

    Junsu Kang

    Insert

    lifeact-mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. linker-P2A-mNeongreen-ORF1

    Plasmid

    #241176

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. linker-P2A-mNeongreen-ORF2

    Plasmid

    #241177

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. linker-P2A-mNeongreen-ORF3

    Plasmid

    #241178

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mNeongreen

    Depositor

    Junsu Kang

    Insert

    mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRT-acrymCherry-FRT

    Plasmid

    #241179

    Purpose

    Middle entry vector for mini-Golden subcloning platform; acry-mCherry

    Depositor

    Junsu Kang

    Insert

    acry-mCherry
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROPseq-multi-EBFP2-NLS (pRW1447)

    Plasmid

    #216219

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge