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pM9_fcpA_NI

Plasmid

#90418

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NI

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pM9_NR_NI

Plasmid

#90422

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NI

Depositor

Peter Kroth

Publication

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_2

Plasmid

#26718

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Tuba_2 RNAi

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pPdiso4E-ISH

Plasmid

#61701

Purpose

Plum eIF(iso)4E ihp-RNA construct

Depositor

Lining Tian

Publication

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF(iso)4E

Use

RNAi

Promoter

35S

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215328

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Publication

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

FOXJ1 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

FOXJ1

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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CMV5.1-SARS-CoV-2 Nsp3

Plasmid

#204205

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Publication

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx3

Expression

Mammalian

Mutation

none, from obtained clone

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

Plasmid

#20174

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (K83A,D84N,G86D) in the EF hand of ST…

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Publication

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nano-lantern(cAMP-0.4)/pRSETB

Plasmid

#53590

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Tags

6xHis

Expression

Bacterial

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
C-Fos scFv [N486/32]

Plasmid

#182090

Purpose

Mammalian Expression of C-Fos scFv. Derived from hybridoma N486/32.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting C-Fos (Homo sapiens) (FOS Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCNPpo6-H98A

Plasmid

#46087

Depositor

Raymond Monnat

Publication

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Insert

PpoI H98A

Tags

NLS

Expression

Mammalian

Mutation

catalytically inactive H98A variant

Promoter

CMV

Available since

June 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV6- Entry-RPL26DC-DDK

Plasmid

#133867

Purpose

Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26DC-myc-FLAG (RPL26 Human)

Tags

Myc-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Publication

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA E171Q GFP

Plasmid

#32127

Depositor

Susan Lindquist

Publication

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

E171Q

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Publication

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-CBM-CohI

Plasmid

#58709

Purpose

Probing molecule to perform single molecule force spectroscopy experiments

Depositor

Hermann Gaub

Publication

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

CBM-CohI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Alanine 2 to Cysteine in CBM domain

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCherryC1 VAP-A KD/MD

Plasmid

#226408

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-A (VAPA Human)

Tags

T7-Xpress tag and mCherry

Expression

Mammalian

Mutation

K94D/M96D mutant, unable to bind FFAT motifs

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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LRP4 (extracellular) scFv [N207/27]

Plasmid

#190537

Purpose

Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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