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pPU-HSA-miR30-L1221

Plasmid

#174221

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-MDA5

Plasmid

#174222

Purpose

All-in-one control rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdCFID

Plasmid

#221909

Purpose

Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaCFID (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of the CBP-FUS interaction domain (CFID)…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT56

Plasmid

#218727

Purpose

Expresses N-terminally EGFP-tagged IFT56 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nozaki et al J Cell Sci. 2016 Dec 7. pii: jcs.197004.

Insert

IFT56 (TTC26 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMBD2.1R (pc2813)

Plasmid

#211575

Purpose

Expresses MBD2 (aa1-152) RFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (APEX)

Plasmid

#213406

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-2

Plasmid

#204433

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K614A H615A D835A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-9

Plasmid

#204440

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E213A Q217A R557A D565A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-KATNA1, V55D

Plasmid

#180651

Purpose

Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GGACAGCACUCCCUUGAAA

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCENPBR pc861

Plasmid

#167569

Purpose

Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

Homo sapiens centromere protein B (CENPB Human)

Tags

Ds-Red

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-gephyrin C4a

Plasmid

#199611

Purpose

Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

C4a casette insertion

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-TdIF2

Plasmid

#196906

Purpose

visualization of TdIF2/ERBP as a fusion to mCherry

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TdIF2

Plasmid

#199699

Purpose

visualization of nucleolus in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-QQN

Plasmid

#193563

Purpose

Expresses human Clathrin Light Chain B mutant (residues 20-22 were substituted from EED to QQN) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Changed from EED to QQN at residue 20-22

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRBx2

Plasmid

#193569

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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