164,577 results

1. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3962 pcDNA3-IRF3-FL

   Plasmid

   #22860

   Depositor

   Peter Howley

   Article

   Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

   Insert

   IRF3 (IRF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. plenti hSyn PKM1

   Plasmid

   #220920

   Purpose

   Neuronal specific expression of PKM1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PKM1 (PKM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

   Plasmid

   #174665

   Purpose

   Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

   Depositor

   Eli Bar

   Inserts

   LUC2
   mCherry:T2A:Hygro
   

   Use

   Lentiviral and Luciferase

   Tags

   mCherry and hygromycin dual reporter gene linked …

   Expression

   Mammalian

   Mutation

   Promoter

   Human cytomegalovirus immediate early enhancer/pr…

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lambda Phosphatase

   Plasmid

   #79748

   Purpose

   Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   Lambda Phosphatase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-rab11 WT

   Plasmid

   #12674

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NW11

   Plasmid

   #133443

   Purpose

   Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEYFP-C1-Drp1

   Plasmid

   #45160

   Depositor

   Richard Youle

   Article

   Frank et al Dev Cell. 2001 Oct;1(4):515-25.

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60230

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1-SopF

    Plasmid

    #137734

    Purpose

    Expresses EGFP fused to SopF

    Depositor

    Leigh Knodler

    Insert

    SopF
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge