We narrowed to 17,075 results for: sta
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Plasmid#177088PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)DepositorInsertP19, CrDXS2, PaGPPS1, and CrGES
UseSynthetic BiologyAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F)
Plasmid#136609PurposeExpresses GB1 WT mutant (A34F)DepositorInsertGB1 WT mutant (A34F)
Tags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34FPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEE
Plasmid#159748PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorPublicationInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K214R
Plasmid#127132DepositorAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB005
Plasmid#119678PurposeCoding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertgeneticin resistance marker
UseSynthetic Biology; Domestication of dna parts for…Available sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB035a
Plasmid#79190PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
ExpressionBacterialPromoterPlac62; PtetAvailable sinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
ExpressionPlantPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V
Plasmid#106184PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterhSynapsinAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A
Plasmid#106191PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-FLAG
Plasmid#105783PurposeExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-FLAGExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPDK3214
Plasmid#97406Purposebinary construct of ER-targeted GUS with sfCherry11 tag for plant expressionDepositorInsertER-GUS-sfCherry11
TagsER targetExpressionPlantAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.4.1
Plasmid#97363PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHCV E1E2
ExpressionMammalianAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Tomato MEF2C S222A
Plasmid#89716PurposeRetroviral gene expression vector for MEF2C-S222A expressionDepositorAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-2
Plasmid#87333PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) Tac-delta scr
Plasmid#80019PurposeMammalian expression of Tac-delta scrDepositorInsertTac (IL2RA Human)
TagsKLRFGFKExpressionMammalianMutationCD25 amino acids 1 to 263 fused to KLRFGFKPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only