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pVF25

Plasmid

#229457

Purpose

Expresses IraD with R67A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (R67A)

Tags

6xHis

Expression

Bacterial

Mutation

R67A

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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JD73

Plasmid

#229482

Purpose

Adenoviral VA RNA I & II genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA I & II

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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JD143

Plasmid

#229492

Purpose

CMV promoter driving expression of adenoviral E4orf6-6/7 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4orf6-6/7

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD81

Plasmid

#229481

Purpose

Adenoviral VA RNA II gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA II

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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JD92

Plasmid

#229478

Purpose

CMV promoter driving expression of adenoviral E4orf6 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4orf6

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGGA016

Plasmid

#228231

Purpose

Level 0 for 5xOp (GreenGate A module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

5xOp

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGGC018

Plasmid

#228232

Purpose

Level 0 for LhG4 (GreenGate C module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

GR-LhG4

Use

Synthetic Biology

Promoter

-

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_reporter_vector

Plasmid

#221460

Purpose

Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

gypsy insulator-EGFP-neutral spacer DNA-mCherry

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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