Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,781 results for: construct

Clear keywords
Showing: 8661 - 8680 of 8781 results

  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

    Enlarge

  4. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

    Enlarge

  5. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55776-plasmid-map-sequence-id-112541

    Enlarge

  8. Fibronectin-human-mutated V1 O-Glycosylation site

    Plasmid
    #120406
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120406-plasmid-map-sequence-id-233165

    Enlarge

  9. pLEX307-RAD54L2-D1315-1467-SFB

    Plasmid
    #247919
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1315-146
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 1315-1467
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247919-plasmid-map-sequence-id-495273

    Enlarge

  10. pLEX307-RAD54L2-D87-291-SFB

    Plasmid
    #247914
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D87-29
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 87-291
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    247914-plasmid-map-sequence-id-494863

    Enlarge

  11. pLEX307-RAD54L2-D1-86-SFB

    Plasmid
    #247913
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1-86
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 1-86
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    247913-plasmid-map-sequence-id-494860

    Enlarge

  17. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231237-plasmid-map-sequence-id-459172

    Enlarge

  18. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231235-plasmid-map-sequence-id-459171

    Enlarge

  19. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231236-plasmid-map-sequence-id-459169

    Enlarge

Showing: 8661 - 8680 of 8781 results