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pDONR223_TBK1_WT

Plasmid

#82285

Purpose

Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBK1 (TBK1 Human)

Use

Gateway Cloning

Mutation

N388D; K570Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-TNK1

Plasmid

#23894

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TNK1 (TNK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ALPK1

Plasmid

#23630

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALPK1 (ALPK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MMLVgag-3NES-Cre

Plasmid

#207252

Purpose

Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MMLVgag-3NES-Cre

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX PuroR

Plasmid

#166228

Purpose

Lentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

mCherry-CAAX

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_rtTA-P2A-BFP_HygroR

Plasmid

#186738

Purpose

rtTA-P2A-BFP expression construct cloned into lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

rtTA

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAV1 for Split-PE, SpCas9 nickase (PKC1001)

Plasmid

#190111

Purpose

AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

AAV

Tags

bpNLS

Mutation

H840A

Promoter

EF1a

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNA2D2_OE_1

Plasmid

#161616

Purpose

Overexpresses CACNA2D2

Depositor

Mike McManus

Insert

CACNA2D2 (CACNA2D2 Human)

Tags

V5

Expression

Mammalian

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR223 CD147, open

Plasmid

#149720

Purpose

for Gateway cloning of CD147 into destination vector

Depositor

Anne-Claude Gingras

Insert

CD147 (BSG Human)

Use

Gateway Cloning

Mutation

wt, without stop codon

Available Since

June 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ZJOM151

Plasmid

#133643

Purpose

Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-COX4-GFP (COX4 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM153

Plasmid

#133642

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

Vph1-2GFP (VPH1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A5

Plasmid

#132007

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A5 (SLC25A5 Human)

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAG384 bFos-CFAST11

Plasmid

#130815

Purpose

Expresses bFos-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bFos-CFAST11

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKEW189-MBP-TEV-AcrVA1

Plasmid

#115660

Purpose

Expresses AcrVA1 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_8

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-I3-01-Myc

Plasmid

#80038

Purpose

Expression of I3-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

I3-01-Myc

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB110

Plasmid

#68564

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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ecALDH

Plasmid

#67921

Purpose

Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

Depositor

Justin Siegel

Insert

Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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