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pmCherry-PH(Btk)KD(Akt1)

Plasmid

#86636

Purpose

Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)KD(Akt1)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNR163

Plasmid

#79544

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNR165

Plasmid

#79646

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR187

Plasmid

#79553

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Tma16

Plasmid

#63779

Purpose

yeast ORF Tma16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Tma16 (TMA16 Budding Yeast)

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR164

Plasmid

#79545

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL00002

Plasmid

#50347

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Mutation

A206K

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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TAL-2AA3

Plasmid

#41480

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AA3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AC3

Plasmid

#41481

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AC3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AG3

Plasmid

#41482

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AG3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AT3

Plasmid

#41483

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AT3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2CA3

Plasmid

#41484

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2CA3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2CC3

Plasmid

#41485

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2CC3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only