We narrowed to 18,493 results for: Met

1. pRU54

   Plasmid

   #167680

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PXWABS62(E11-TEV)

   Plasmid

   #123157

   Purpose

   For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   ParsR-ABS62, Pecf11

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLHDR02_EGFP_tCD8

   Plasmid

   #124061

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   EGFP
   tCD8
   

   Use

   Mouse Targeting; Human targeting

   Promoter

   Human UbC promoter modified

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EFF-1::V5

   Plasmid

   #132961

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   EFF-1::V5
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.Syn-FLEX.iGABASnFR.mRuby3

   Plasmid

   #112169

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR
   

   Use

   AAV

   Tags

   mRuby3

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER2-mGreenLantern

   Plasmid

   #186548

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   mGreenLantern
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C'5802

   Plasmid

   #176879

   Purpose

   High constitutive expression of Firefly Luciferase in plants

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Insert

   AtUbi10:Luc+:RbcsE9
   

   Use

   Luciferase

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-BRAT1

   Plasmid

   #62802

   Purpose

   Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

   Depositor

   Toru Ouchi

   Article

   So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

   Insert

   BRAT1 (BRAT1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. As4

    Plasmid

    #123161

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J115-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J115, ParsR

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD017-39 TRAC PDCD1 AAV double KO AAV

    Plasmid

    #192153

    Purpose

    TRAC PDCD1 AAV double KO AAV

    Depositor

    Sidi Chen

    Article

    Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

    Insert

    sgRNA
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ER2-LSSmGFP

    Plasmid

    #186538

    Purpose

    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR-mClover3

    Plasmid

    #228605

    Purpose

    Mammalian expression of mClover3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pemiRFP703-myosin

    Plasmid

    #136565

    Purpose

    LC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP703-myosin
    

    Tags

    emiRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. WZLneo-C/EBPα

    Plasmid

    #34567

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    C/EBPα (Cebpa Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Promoter

    EFs

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS-H2B-EYFP

    Plasmid

    #53746

    Purpose

    contains fusion of human histone H2B to EYFP in pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pmiRFP713-tubulin

    Plasmid

    #136561

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP713-tubulin
    

    Tags

    miRFP713

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dendra2-LaminB1-10

    Plasmid

    #57728

    Purpose

    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pRU206

    Plasmid

    #167693

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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