We narrowed to 8,865 results for: APP
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Plasmid#203895PurposeDAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertDAT promoter and 5'UTR
ExpressionPlantMutationMutation of BpiI or BsaI cut site for MoClo domes…Available SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)
Plasmid#198072PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182682PurposeContains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.DepositorInsertmouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterNAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
TKIT NFL gRNA1 gRNA2
Plasmid#182681PurposeExpression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.DepositorInserttwo Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
UseCRISPRExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsExpressionMammalianPromoterCMV and NAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem-GFP
Plasmid#162501PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.DepositorTagsGFPExpressionMammalianMutationThe Tac stem region is inserted within ST6Gal1Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-31-192-193a-Reporter
Plasmid#71874PurposeMammalian expression vector for the analysis of miR-31-19α-193a activityDepositorInsertBinding sequence targeted by miR-31, miR192, and miR-193a-5p
Tagsfirefly luciferaseExpressionMammalianPromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
Tagsfirefly luciferaseExpressionMammalianPromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-193a-5p-Reporter
Plasmid#71873PurposeMammalian expression vector for the analysis of miR-193a-5p activityDepositorInsertReverse complementary sequence of miR-193a-5p
Tagsfirefly luciferaseExpressionMammalianPromoterCMVAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
JWW-1 human chimeric antibody
Plasmid#66748PurposeExpresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.DepositorInsertsJWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa
ExpressionMammalianMutationFirst 3 amino acids of rat light chain constant r…PromotermEF1 and rEF1Available SinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPRK1-DuET
Plasmid#213359PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PuroR-P2A-DD-TCOF1-donor
Plasmid#60747PurposeDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
BlastR-P2A-DD-TCOF1-donor
Plasmid#60748PurposeDonor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDA_122
Plasmid#208095PurposePerturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seqDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEF1aAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Mouse, Human)
TagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUN1213 - pL0_p16OMT (pro + 5U)
Plasmid#203890Purpose16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsert16OMT promoter and 5'UTR
ExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …Available SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1214 - pL0_pNMT (pro + 5U)
Plasmid#203893PurposeNMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertNMT promoter and 5'UTR
ExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …Available SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ERAP1-rs27895(C)
Plasmid#206142PurposeExpresses the rs27895-C allele of ERAP1, myc-tagged.DepositorInsertERAP1 (ERAP1 Human)
Available SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro
Plasmid#198845PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-R18 TALEN
ExpressionMammalianAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro
Plasmid#198841PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-R15 TALEN
ExpressionMammalianAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only