164,577 results

1. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fuw-dCas9-Tet1CD

   Plasmid

   #84475

   Purpose

   Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEHIShqprt

   Plasmid

   #92166

   Purpose

   Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

   Depositor

   James Naismith, Huanting Liu

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   Quinolinate Phosphoribosyltransferase (QPRT Human)
   

   Use

   Tags

   TEV protease -cleavable N-terminal 6His

   Expression

   Bacterial

   Mutation

   changed Asp 2 to Asn to create BspHI site

   Promoter

   T7

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-CAGGS-dCas9-KRAB

   Plasmid

   #110822

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-NLS-HA-Dre

   Plasmid

   #51272

   Purpose

   Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   Dre
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

   Plasmid

   #226574

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   R119A, F191A

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

   Plasmid

   #125256

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

   Depositor

   Roger D. Kamm

   Article

   Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

   Insert

   hChR2(H134R)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-forward

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lact-C2-GFP

   Plasmid

   #22852

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 Domain (MFGE8 Bovine)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINF

   Plasmid

   #26042

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His3C-lacZ
   

   Use

   Tags

   MAHHHHHHSSGLEVL

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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