We narrowed to 9,182 results for: Pol

1. pEXP5-NT-7th R

   Plasmid

   #42573

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated calmodulin gene
   

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

   Plasmid

   #177887

   Purpose

   Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Tags

   10xHis-TEV-4xFRB-SNAP tag

   Expression

   Insect

   Mutation

   G2A

   Promoter

   Polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Promoter

   EF1 alpha

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV4-ApoE3

   Plasmid

   #87086

   Purpose

   Expresses ApoE3 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E3 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIV-EGFP.Flag-EZH2

   Plasmid

   #220243

   Purpose

   Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Tags

   His

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4-ApoE4

   Plasmid

   #87087

   Purpose

   Expresses ApoE4 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E4 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HAP40 1-371

    Plasmid

    #124060

    Purpose

    Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HAP40 (F8A1 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Promoter

    polyhedrin

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-MYO7A-TAIL

    Plasmid

    #89585

    Purpose

    Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO7A (Myo7a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation encoding amino acids 870-2166 of MYO7A…

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB1.HMBP.PrS.MTF2

    Plasmid

    #125169

    Purpose

    Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    MTF2 (MTF2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 TAP DHX36

    Plasmid

    #53547

    Purpose

    mammalian expression of DHX36

    Depositor

    Kathleen Collins

    Article

    Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    TAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-hCD155

    Plasmid

    #118630

    Purpose

    human CD155 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    CD155 (PVR Human)
    

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Promoter

    TEF1p

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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