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pGEX-4T-1-Rnt1-dsRBD(+3A)

Plasmid

#171551

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a three-alanine insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD(+6)

Plasmid

#171550

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Dicer-dsRBD (+6)

Plasmid

#171544

Purpose

Encodes a Dicer fragment extending from from R1841 to the C-terminus

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DICER1 (DICER1 Human)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Rnt1-dsRBD

Plasmid

#171549

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-DGCR8-2xdsRBD(+6)

Plasmid

#171542

Purpose

Encodes a DGCR8 fragment extending from E502 to the C-terminus

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Staufen-dsRBD(+6)

Plasmid

#171546

Purpose

Encoding part of the Drosophila Staufen protein from M571 to P656

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Staufen (stau Fly)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Staufen

Plasmid

#171545

Purpose

Encoding part of the Drosophila Staufen protein from M571 to P656

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Staufen (stau Fly)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mRplp1

Plasmid

#173153

Purpose

mouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al Mol Metab. 2024 Sep;87:101997. doi: 10.1

Insert

ORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)

Tags

His

Expression

Mammalian

Mutation

Wild type

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pKB237.1

Plasmid

#170031

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTub-alpha-1-mtGFP

Plasmid

#138981

Purpose

Neuron-specific expression of membrane targeted EGFP

Depositor

Frank Bradke, Sebastián Dupraz

Article

Santos et al Cell Rep. 2020 Jul 21;32(3):107907. doi:

Insert

EGFP

Expression

Mammalian

Mutation

EGFP (added membrane targeting domain from GAP43).

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

Plasmid

#166993

Purpose

Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0), Hairpin = 0 nt

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1, PolyU(0)

Plasmid

#166992

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Nup54 no FG-mEGFP494(1)

Plasmid

#164270

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Article

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(1)-CRY2-mCherry

Plasmid

#160440

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-201-2xStrep

Plasmid

#138442

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-201

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 1-226-2xStrep

Plasmid

#138443

Purpose

Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids 1-226

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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