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MCV

Plasmid

#81174

Purpose

MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSK-c-mpl

Plasmid

#1828

Depositor

Philip Leder

Article

Skoda et al EMBO J 1993 Jul;12(7):2645-53.

Insert

c-mpl (Mpl Mouse)

Expression

Bacterial

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Xba1

Plasmid

#203300

Purpose

For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4C01

Plasmid

#71009

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cand1 (CG5366 Fly)

Use

Gateway Cloning

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pRK DPC4 deltaC Flag

Plasmid

#12628

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 delta C (SMAD4 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated DPC4 (aa 1-511)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or9a

Plasmid

#59637

Purpose

Probe for Drosophila melanogaster Or9a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or9a (Or9a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
HNRNPA1_Halo_C_allele

Plasmid

#178123

Purpose

Donor vector for endogenous tagging of human HNRNPA1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (HNRNPA1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Ngn3 Cre (DM#259)

Plasmid

#15023

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
ADAM10_Halo_C_allele

Plasmid

#178151

Purpose

Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ADAM10 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-KLF4

Plasmid

#35389

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Klf4

Use

Lentiviral

Expression

Mammalian

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
p70rg 15

Plasmid

#17831

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pLOX-EWgfp

Plasmid

#12242

Depositor

Didier Trono

Article

Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

EF1a promoter is upstream of GFP. LoxP in 3'…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGEM-T Dmel Or35a

Plasmid

#59650

Purpose

Probe for Drosophila melanogaster Or35a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or35a (Or35a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
Ngn3 Cre-ER (DM#256)

Plasmid

#15024

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pCWX-R4-DEST-R2-PGR65

Plasmid

#45953

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only