We narrowed to 3,085 results for: multiplex

1. pScI_dCas9-CDA_J23119-sgRNA

   Plasmid

   #108550

   Purpose

   Bacterial Target-AID vector with sgRNA

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Inserts

   dCas9-PmCDA1
   Streptococcus pyogenes sgRNA
   

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   J23119 and lambda OR

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163076

   Purpose

   Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRU295

   Plasmid

   #167694

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Plasmid

   #163075

   Purpose

   Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   roGFP2-Orp1
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYL-kasOp-FnCas12a3

   Plasmid

   #177875

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized the FnCas12a mutant EP16
   

   Use

   CRISPR

   Promoter

   kasOp*

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

   Plasmid

   #192651

   Purpose

   3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-10xGCN4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RNA-Protein Landing Pad

   Plasmid

   #226162

   Purpose

   Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   mTagBFP2-P2A-iCasp9
   Reverse tetracycline transactivator 3
   Hygromycin-IRES-EGFP
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF464

    Plasmid

    #167672

    Purpose

    Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-dCas9 VP64-T2A-GFP

    Plasmid

    #53192

    Purpose

    Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Salsa-BC96-P1H12

    Plasmid

    #202937

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC96

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC96
    

    Expression

    Mammalian

    Promoter

    Tet Response Element (TRE)

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCMV Puro DEST P38KTRDronpa

    Plasmid

    #205763

    Purpose

    Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    P38 KTR Dronpa
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

    Plasmid

    #175539

    Purpose

    Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ131C

    Plasmid

    #69284

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ132C

    Plasmid

    #69285

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV/MASC_(GLuc)_TOP1

    Plasmid

    #68439

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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