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Plasmid#114067PurposeT7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guidesDepositorInsertentry vector for AsCas12a crRNAs
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-mKO-MK2
Plasmid#115492PurposeExpression vector for the p38 activity reporter mKO-MK2 in mammalian cellsDepositorInsertmKO-MK2 (MAPKAPK2 Human)
UseTagsmonomeric Kusabira Orange (mKO)ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (1-249)
Plasmid#42204DepositorInsertc-SRC (1-249) (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 1-249PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548e-5p
Plasmid#103634PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548e-5p target (MIR548E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Human, Synthetic)
UseTagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (1-397)
Plasmid#42205DepositorInsertc-SRC (1-397) (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 1-397PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHIV-INK4A-zsGreen
Plasmid#110728PurposeMammalian Expression of p16INK4A IRES zsGreenDepositorInsertp16INK4A IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30d-5p
Plasmid#103417PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30d-5p target (MIR30D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-132-3p
Plasmid#103219PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-132-3p target (MIR132 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-21-5p target (MIR21 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p323.hSyn.Halo-mFMRP.Wpre
Plasmid#185909PurposeExpresses FMRP fused to HaloTag for protein localization through fluorescence imagingDepositorInsertHaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterhSyn (human Synapsin promoter)Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV1
Plasmid#74981PurposeConstitutive ETV1 ORF, blasticidin selectionDepositorInsertETV1 (ETV1 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorInsertETV4 (ETV4 Human)
UseTagsExpressionMammalianMutationS396NPromoterEF1aAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-27a-3p
Plasmid#103381PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-27a-3p target (MIR27A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133b
Plasmid#103223PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133b target (MIR133B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1, transient)
Plasmid#197588PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA
Plasmid#194377PurposeSplit TEV assaysDepositorInsertSP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-3p
Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-zsGreen
Plasmid#110729PurposeMammalian Expression of p14ARF IRES zsGreenDepositorInsertp14ARF IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA
Plasmid#194381PurposeSplit TEV assaysDepositorInsertSP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)
Plasmid#114068PurposeT7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guidesDepositorInsertentry vector for LbCas12a crRNAs
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (91-536)
Plasmid#42206DepositorInsertc-SRC (91-536) (SRC Human)
UseTagsExpressionMammalianMutationcontains amino acids 91-536PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBG-bZIPa
Plasmid#12436DepositorInsertbZIP of C/EBPa (CEBPA Human)
UseTagsGSTExpressionMammalianMutationpEBG vector is from Bruce Mayer, may be different…PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-S
Plasmid#74983PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorInsertETV4 (ETV4 )
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30a-3p
Plasmid#103409PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30a-3p target (MIR30A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-296-3p
Plasmid#103387PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-296-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-296-3p target (MIR296 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-146a-3p
Plasmid#103247PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-146a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-146a-3p target (MIR146A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-3p
Plasmid#103145PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-3p target (MIRLET7A3 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
nes714tk/lacZ
Plasmid#47614Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZExpressionMutation714 bp from 3' end of 2nd intronPromoterHSV tk promoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only