We narrowed to 1,336 results for: cbs

1. p5E-hs_ICAM_full (JDW 1047)

   Plasmid

   #224479

   Purpose

   Gateway compatible 5' entry clone containing the Human ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   ICAM2 promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME Actin Vhh Halo Tag (JDW 1223)

   Plasmid

   #224498

   Purpose

   Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-Halo-Tag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JE301: pMVP (L5-L4) Tir1-P2A-AID

   Plasmid

   #121722

   Purpose

   pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1-P2A-AID (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc (C-term on osTIR1)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. P119 C-Check

   Plasmid

   #66817

   Purpose

   Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

   Depositor

   Yonglun Luo

   Article

   Zhou et al Cell Mol Life Sci. 2016 Jan 11.

   Insert

   truncated Enhanced Green Fluorescent Protein and asRED
   

   Expression

   Mammalian

   Promoter

   PGK and CMV

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

   Plasmid

   #224514

   Purpose

   A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xNLS-(G2SG2)2-Cre
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LENTICRISPR-HK2_sgRNA1

   Plasmid

   #201596

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p5E-TetO8x-CMVmin (JDW 1087)

   Plasmid

   #224475

   Purpose

   Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TetO(8x)-CMV promoter
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWPI-LUC7L-3xFLAG

   Plasmid

   #201640

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EIF4G2_MIF4G_pGEX

    Plasmid

    #135138

    Purpose

    Recombinant expression of EIF4G2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    EIF4G2 (EIF4G2 Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid

    #121831

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121814

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH-eCFP

    Plasmid

    #176555

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL-eCFP

    Plasmid

    #176556

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of leucine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU-eCFP

    Plasmid

    #176557

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU-mRuby2

    Plasmid

    #176549

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH-mRuby2

    Plasmid

    #176547

    Purpose

    Expression of mRuby2 for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    yomRuby2
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge