We narrowed to 2,075 results for: pam

1. pHEE901-A3A

   Plasmid

   #229044

   Purpose

   nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL436 HEK293site3EQR sgRNA

   Plasmid

   #126445

   Purpose

   To cut the human HEK293 site3 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human HEK293 site3 with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p35S::SISOBIR1-YFPc

   Plasmid

   #86164

   Purpose

   SOBIR1 split YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   YFPc

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p35S::AtSOBIR1-YFPn

   Plasmid

   #86165

   Purpose

   SOBIR1 split YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Use

   Tags

   YFPn

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2T-FRB-W2027F

   Plasmid

   #73341

   Purpose

   Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
   

   Use

   Tags

   GST-tagged

   Expression

   Bacterial

   Mutation

   FRB domain only aa2015-2114; W2027F

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-2T FRB Y2105A

   Plasmid

   #68726

   Purpose

   Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
   

   Use

   Tags

   GST-tagged

   Expression

   Bacterial

   Mutation

   FRB domain only aa2015-2114; Y2105A

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

7. VE-cad KI gRNA1

   Plasmid

   #92310

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA1 (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. VE-cad KI gRNA2

   Plasmid

   #92311

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA2 (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AA025

   Plasmid

   #216005

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-NG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs_FLAG-MCART1

    Plasmid

    #133247

    Purpose

    Express FLAG-tagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AA180

    Plasmid

    #216023

    Purpose

    Fragmid fragment: (Cas protein) NGRR(T) PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sa]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AA027

    Plasmid

    #216007

    Purpose

    Fragmid fragment: (Cas protein) nearly PAM-less

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpRY_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AA026

    Plasmid

    #216006

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXs_MCART1

    Plasmid

    #133253

    Purpose

    Express untagged MCART1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells and…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid

    #190190

    Purpose

    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed EGFP Kozak sequence inserting a C>T mo…

    Promoter

    EIF1-short

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. JAM1-CD4d3+4-bio

    Plasmid

    #73110

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding JAM1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    JAM1 (F11R Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AA007

    Plasmid

    #216002

    Purpose

    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV_Npu-saBE3.9max_KKH C-terminal

    Plasmid

    #137181

    Purpose

    Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    Npu-saBE3.9max_KKH_C-terminal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    CMV

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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