We narrowed to 5,093 results for: puc

1. pUCIDT-attL1-Worm ABeta-attR5

   Plasmid

   #160437

   Purpose

   Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
   

   Tags

   Cleavage signal

   Expression

   Bacterial

   Mutation

   Codon-optimized for expression in C. elegans

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

   Plasmid

   #110554

   Purpose

   miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Expression

   Bacterial

   Promoter

   AraC-ParaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

   Plasmid

   #98971

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNA and spCas9
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-hph-Ptet1

   Plasmid

   #218367

   Purpose

   Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-hph-dCas9

   Plasmid

   #218371

   Purpose

   Expression of dCas9 for CRISPR-interference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PtetA

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-hph-Ptac

   Plasmid

   #218364

   Purpose

   Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

   Plasmid

   #129537

   Purpose

   PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   tetR-3xFlag-HA-2A-Puro
   

   Use

   Synthetic Biology

   Promoter

   hPGK promoter

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18T-mini-Tn7T-hph-Para

   Plasmid

   #218363

   Purpose

   Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-DEST-hPROX1-tdTomato-PuroTK

    Plasmid

    #141027

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BsaI-tagRFP-BsaI-pUC-kan

    Plasmid

    #170771

    Purpose

    test plasmid for Golden Gate assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Promoter

    none

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18T-mini-Tn7T-Zeo-dsRedExpress

    Plasmid

    #65040

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pUC18T-mini-Tn7T-Zeo-eyfp

    Plasmid

    #65039

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIG-730pUC19-tNGFR-P2A-Coronin1A

    Plasmid

    #186110

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CORONIN1A
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCIDT-attL1-Human ABeta-attR5

    Plasmid

    #160436

    Purpose

    Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC18T-mini-Tn7T-Zeo-ecfp

    Plasmid

    #65038

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    ecfp
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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