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We narrowed to 928 results for: trna

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Showing: 861 - 880 of 928 results

  2. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  7. pUltra-sY

    Plasmid
    #82417
    Purpose
    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Methanococcus jannaschii aaRS for sulfotyrosine
    Expression
    Bacterial
    Mutation
    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…
    Promoter
    tacI
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    82417-plasmid-map-sequence-id-156040

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  8. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  10. pSC4

    Plasmid
    #91182
    Purpose
    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    gRNAs targeting 58kb region in medicago truncatula
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91182-plasmid-map-sequence-id-182722

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  11. pBBR1-EcTrpRS(H14)

    Plasmid
    #231131
    Purpose
    Expresses mutant E. coli TrpRS from a derepressed lac promoter for proteome-wide incorporation of tryptophan analogues in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tryptophanyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    S8A, V144G, V146C
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231131-plasmid-map-sequence-id-458351

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  12. pBBR1-EcTyrRS(VSMA,D165G)

    Plasmid
    #231132
    Purpose
    Expresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae.
    Depositor
    Abhishek Chatterjee
    Article
    Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/
    Insert
    E. coli tyrosyl tRNA synthetase
    Use
    Klebsiella pneumoniae expression
    Expression
    Bacterial
    Mutation
    Y37V, D165G, D182S, P183M, L186A, D265R
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    231132-plasmid-map-sequence-id-458349

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  18. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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Showing: 861 - 880 of 928 results