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pCAG-PDZD8HA_sh_resistant

Plasmid

#105006

Purpose

Mammalian Expression of PDZD8, sh resistant

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Mutation

The sequence recognized by shPDZD8 (TRCN000043105…

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB128

Plasmid

#68582

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Article

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179119

Purpose

Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Ezr sgRNA

Use

AAV

Promoter

U6

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact EGFP

Plasmid

#187686

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OT1.0

Plasmid

#185384

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

Glucagon-ICAM1-pre-mGRASP

Tags

3x HA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM75

Plasmid

#133649

Purpose

Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-ANP1-mCherry (ANP1 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-JAG1-E228K

Plasmid

#116420

Purpose

Lentiviral expression of JAG1 E228K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

JAG1 (JAG1 Human)

Use

Lentiviral

Mutation

E228K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-miRFP

Plasmid

#108416

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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NRIP1_pCSdest

Plasmid

#53805

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

NRIP1 (NRIP1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MSP2283

Plasmid

#70702

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-N 18E6

Plasmid

#37883

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 18E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-2µΔ13V

Plasmid

#28000

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-2µΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

H152A

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only
CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only