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pFUW-tetO-Pu.1

Plasmid

#139839

Purpose

doxycycline-inducible expression of mouse Pu.1 in mammalian cells

Depositor

Filipe Pereira

Publication

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Pu.1 (Spi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Publication

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Publication

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2 hSmad1-WT

Plasmid

#22989

Depositor

Edward De Robertis

Publication

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Publication

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Expression

Bacterial

Mutation

None, WT gene

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSG5 PMS2-wt

Plasmid

#16475

Depositor

Bert Vogelstein

Publication

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

PMS2 (PMS2 Human)

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-155

Plasmid

#118569

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Publication

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Publication

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Dynamitin-GFP

Plasmid

#51419

Purpose

expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

Depositor

Trina Schroer

Publication

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

Dynamitin (DCTN2 Chicken)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Publication

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Publication

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA4-Flag-XPA

Plasmid

#22895

Depositor

Aziz Sancar

Publication

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

XPA (XPA Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CF-iSH

Plasmid

#20159

Depositor

Tobias Meyer

Publication

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-p85 (Pik3r2 Mouse)

Expression

Mammalian

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLPC TRF2

Plasmid

#18002

Depositor

Titia de Lange

Publication

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Mutation

aa2-500

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
c-myc promoter (4x TBE2 - wt)

Plasmid

#16599

Depositor

Bert Vogelstein

Publication

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pRK7-Rheb

Plasmid

#15888

Depositor

John Blenis

Publication

Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

Insert

Rheb (RHEB Human)

Tags

Flag

Expression

Mammalian

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles