We narrowed to 16,586 results for: EMB

1. Endolyn-delta pMEP4

   Plasmid

   #62962

   Purpose

   full length rat Endolyn cloned into delta pMEP4

   Depositor

   Paul Luzio

   Article

   Ihrke et al Biochem J. 2000 Jan 15;345 Pt 2:287-96.

   Insert

   Endolyn (Cd164 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

   Plasmid

   #162949

   Purpose

   Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFX-Venus-KRasCT

   Plasmid

   #175834

   Purpose

   Express Venus with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD_OsU3 (GB1184)

   Plasmid

   #68209

   Purpose

   Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   OsU3 promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pME-hygro-hPGAP3-3HA

   Plasmid

   #50373

   Purpose

   Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5/FRT/TO - RBM28 E5 WT minigene

   Plasmid

   #169269

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E5 WT minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

   Plasmid

   #162918

   Purpose

   Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TB:2xP53_RE:MiniP

   Plasmid

   #133881

   Purpose

   2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-bSV1_1-1009

   Plasmid

   #129471

   Purpose

   Mammalian expression of the N-terminus + central domain of bovine SV1

   Depositor

   Elizabeth Luna

   Article

   Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

   Insert

   Supervillin (SVIL Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   contains amino acids 1-1009

   Promoter

   CMV

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2322

    Plasmid

    #36748

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FOXO1-TALEN_Left (FOXO1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

11. rGSKmyc-R85-pXT7

    Plasmid

    #15493

    Depositor

    Sergei Sokol

    Article

    Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

    Insert

    GSK-3 beta R85 (Gsk3b Rat)
    

    Use

    Xenopus rna expression

    Tags

    myc

    Expression

    Mutation

    R85: K85R, dominant negative

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

12. mCherry-SAC1-HLx8

    Plasmid

    #108139

    Purpose

    SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD3C

    Plasmid

    #86971

    Purpose

    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 fused to phosphite dehydrogenase and P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…

    Promoter

    T7 promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TAL2268

    Plasmid

    #36705

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FOXO3-TALEN_Left (Foxo3 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL2269

    Plasmid

    #36706

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FOXO3-TALEN_Right (Foxo3 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-EGFP-Rheb-HVR HRas-IP

    Plasmid

    #13833

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    Rheb-HVR HRas (Rheb Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminal of Rheb and hyper variable region (HVR…

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pME18S-3HA-mULK2(K39T)

    Plasmid

    #22899

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Oncogene. 1999 Oct 21. 18(43):5850-9.

    Insert

    UNC-51-like kinase 2 (Ulk2 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed Lysine 39 to Threonine

    Promoter

    Available since

    Sept. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-mTAZ-WW (86)

    Plasmid

    #40915

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ 14-3-3 binding site and WW domain (…

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only