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Plasmid#192325PurposeLentiviral expression vector for an inducible 3xFLAG-PFN2_variant2DepositorInsertPFN2_variant2 (PFN2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MTUS1
Plasmid#192298PurposeGateway entry vector for an inducible 3xFLAG-MTUS1DepositorInsertMTUS1 (MTUS1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-NUP37
Plasmid#192299PurposeGateway entry vector for an inducible 3xFLAG-NUP37DepositorInsertNUP37 (NUP37 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-PFN2_variant2
Plasmid#192300PurposeGateway entry vector for an inducible 3xFLAG-PFN2_variant2DepositorInsertPFN2_variant2 (PFN2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-TTI1
Plasmid#192303PurposeGateway entry vector for an inducible 3xFLAG-TTI1DepositorInsertTTI1 (TTI1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPINA7A2mTFP1(F)
Plasmid#193774PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertmTFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPINA7 synthetic promoter carrying binding sites f…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VA
Plasmid#186454PurposepTarget containing protospacer for FnCas12a targetingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VB
Plasmid#186456PurposepTarget containing protospacer for AaCas12b targetingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIT1-2EGFP-ter-URA3
Plasmid#184767PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertCIT1 (CIT1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACH1-2EGFP-ter-URA3
Plasmid#184769PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertACH1 (ACH1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFG3-2EGFP-ter-URA3
Plasmid#184770PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertAFG3 (AFG3 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYS1-2EGFP-ter-URA3
Plasmid#184771PurposeMarker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertSYS1 (SYS1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKC1-2GFP-ter-TRP1
Plasmid#184773PurposeIntegration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertPKC1 (PKC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_SulR(CDS)
Plasmid#186423Purposeprovides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)DepositorInsertSulR
UseSynthetic Biology; Cds (part b3-b4-b5), goldenbra…TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC2
Plasmid#191857PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGA guide 1
Plasmid#193610PurposePIGA knockoutDepositorInsertsgPIGA guide 1 (PIGA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGA guide 2
Plasmid#193611PurposePIGA knockoutDepositorInsertsgPIGA guide 2 (PIGA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only