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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

Plasmid

#192396

Purpose

To act as a 2-input state of the AND gate.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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THOC6

Plasmid

#156085

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC6 (THOC6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RALYL-1

Plasmid

#155877

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RALYL (RALYL Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CIR1

Plasmid

#155452

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CIR1 (CIR1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_UGI-NES_left

Plasmid

#187845

Purpose

expresses the left-side half ND6-UGI-NES in mammalian cells

Depositor

Chengqi Yi

Publication

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CaCYP722C(pYL803)

Plasmid

#178291

Purpose

Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CaCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-LjCYP722C(pYL795)

Plasmid

#178292

Purpose

Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

LjCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-SlCYP722C(pYL1071)

Plasmid

#178294

Purpose

Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

SlCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-TpCYP722C(pYL851)

Plasmid

#178295

Purpose

Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

TpCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-CsCYP722C(pYL877)

Plasmid

#178296

Purpose

Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

CsCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-VvCYP722C(pYL852)

Plasmid

#178297

Purpose

Expresses VvCYP722C from V. vinifera in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VvCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VvCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-GmCYP722C(pYL887)

Plasmid

#178298

Purpose

Expresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GmCYP722C

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-FvCYP722C2(pYL878)

Plasmid

#178299

Purpose

Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

FvCYP722C2

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-OsCYP711A3(pYL761)

Plasmid

#178300

Purpose

Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A3

Expression

Yeast

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.HA.HA.Mm.RAP1

Plasmid

#184642

Purpose

Retroviral expression of mouse RAP1

Depositor

Agnel Sfeir

Publication

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-Zeo

Plasmid

#162740

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Publication

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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