We narrowed to 9,182 results for: Pol

1. tl1-FusXTBE-RightTALE

   Plasmid

   #196507

   Purpose

   Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA
   

   Expression

   Bacterial

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUGP1.1

   Plasmid

   #196615

   Purpose

   Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1_GI.1_A37C-A44C

   Plasmid

   #162582

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Alanine 37 changed to Cysteine and Alanine 44 cha…

   Promoter

   polyhedring

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1_GI.1_Q141V-P221L

   Plasmid

   #162584

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glutamine 141 changed to Valine and Proline 221 c…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1_GI.1_L144C-P221C

   Plasmid

   #162585

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Leucine 144 changed to Cysteine and Proline 221 c…

   Promoter

   polyhderin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1_GI.1_G131C-N172C

   Plasmid

   #162586

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glycine 131 changed to Cysteine and Asparagine 17…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1_GI.1_N167C-L169C

   Plasmid

   #162587

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Asparagine 167 changed to Cysteine and Leucine 16…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJet-CMV-CD52-bglpA

   Plasmid

   #89704

   Purpose

   Human CD52 expression plasmid with short polyA

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CMV-CD52-bglpA (CD52 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-shGBP1.2.mKO2

    Plasmid

    #85210

    Purpose

    TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. miABE

    Plasmid

    #205412

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Bovine, Mouse)
    

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Promoter

    T7 polymerase

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSSRB070_pAC8-hsCRBN

    Plasmid

    #218789

    Purpose

    Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    Protein cereblon (CRBN Human)
    

    Tags

    Flag-TEV-Spy

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. YFP NLS Beta-Actin S14C

    Plasmid

    #60614

    Purpose

    Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    S14C

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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