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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Expression

Mammalian

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Publication

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shPSMD2

Plasmid

#125779

Purpose

constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shPSMD2 (PSMD2 Human)

Use

RNAi

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgCh2-2

Use

CRISPR

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEM4Z globin NB Probe

Plasmid

#120390

Purpose

Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

Depositor

Niels Gehring

Publication

Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

Insert

beta globin partial 3' UTR

Use

Unspecified

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p28atDarpp

Plasmid

#108318

Purpose

Expresses tDarpp in E.coli

Depositor

Jamil Momand

Publication

Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

Insert

tDarpp (PPP1R1B Human)

Tags

6 Histadine

Expression

Bacterial

Promoter

T7

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5080

Plasmid

#85106

Purpose

Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

Depositor

Susan Golden

Publication

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Expression

Bacterial

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-011

Plasmid

#78699

Purpose

A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

Depositor

Brian Chow

Publication

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator

Use

Synthetic Biology

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-009

Plasmid

#78696

Purpose

A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Publication

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BASIC_10_J23101-RBS32-GFP-B0015

Plasmid

#68144

Purpose

storage vector for BASIC part: GFP expression cassette

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS32-GFP-B0015

Expression

Bacterial

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P. falciparum DHPS

Plasmid

#64080

Purpose

Expression of P. falciparum dihydropteroate synthase

Depositor

Vern Schramm

Publication

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Tags

Histidine tag

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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S. aureus DHPS

Plasmid

#64079

Purpose

Expression of Staphylococcus aureus dihydropteroate synthase

Depositor

Vern Schramm

Publication

Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

Insert

dihydropteroate synthase

Tags

Histidine tag

Expression

Bacterial

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pPdiso4E-ISH

Plasmid

#61701

Purpose

Plum eIF(iso)4E ihp-RNA construct

Depositor

Lining Tian

Publication

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF(iso)4E

Use

RNAi

Promoter

35S

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pVRc35_3582

Plasmid

#49750

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS35_3582

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc16_3622

Plasmid

#49738

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS16_3622

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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K-p37

Plasmid

#38789

Depositor

David Wang

Publication

Siebrasse et al J Virol. 2012 Jun 27.

Insert

3754-4585 of MWPyV

Expression

Bacterial

Mutation

1SNP in comparison to WD976

Promoter

lac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-D149A

Plasmid

#29355

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Mutation

Asp149Ala

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only