We narrowed to 17,075 results for: sta
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Plasmid#185628PurposeModified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertlacZ
UseCRISPR and Synthetic BiologyAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 W201E
Plasmid#186471PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationW201E (no more binding to lipid droplets)Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CKAP2-mNeonGreen pHAT
Plasmid#175947PurposeBacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-NQL007-NANOG-sgRNA
Plasmid#175555PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nuclease and sgRNA against mouse NANOG STOP Codon
UseMouse TargetingExpressionMammalianAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Synthetic, Mouse)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-AMBRA1-3xFLAG
Plasmid#174159PurposePiggyBac vector to stably express 3xFLAG-tagged AMBRA1DepositorAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2Δ751-813aa
Plasmid#163603PurposeExpresses BMPR-2 lacking the LIMK-binding region in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe LIMK-binding region (751-813 aa) is deletedAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v3 IQSEC1 WT
Plasmid#162018PurposeExpression of IQSEC1 splice variant 3DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma2
Plasmid#165495PurposeGreen fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
ExpressionMammalianPromoterCMVAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v4 IQSEC1 WT
Plasmid#162019PurposeExpression of IQSEC1 splice variant 4DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2151
Plasmid#141300PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55361
Plasmid#153227PurposeControl plasmid for knockout of AP3 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9, guide RNA for AP3 and FAST marker
UseSynthetic BiologyAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-MAL-SYK
Plasmid#157990PurposeExpresses SYK in insect cell (sf9)DepositorInsertSpleen tyrosine kinase (SYK Human)
TagsMet-Ala-Leu and add Leu,Glu residues +His tagExpressionInsectAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorPublicationInsertsdspAvr2
mCherry
TagsEGFP and HDELExpressionBacterial and PlantPromoter35SAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1A2QS
Plasmid#138268PurposeMINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activityDepositorInsertsAraC-TetR operon
LuxR-LacI operon
mCherry
GFP
UseSynthetic BiologyExpressionBacterialPromoterPara/lac and Plux/tetAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDK480
Plasmid#134197PurposeMammalian expression of Flip in SKAP and IRES GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertuntagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
TagsIRES-GFPExpressionMammalianMutationAlternate Start, G5 siRNA hardenedAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mApple
Plasmid#132566PurposemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmApple
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAD54_CAD29_H104A_pvp008
Plasmid#124876PurposeExpresses hCAD His103Ala mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationmutated His103 to Ala, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2b-srt-his
Plasmid#124804PurposeHDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMurine IgG2b
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only