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pMOD3-Lux-nat

Plasmid

#120323

Purpose

Bacterial luminescence promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

(None)

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-CLIC3

Plasmid

#140583

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CLIC3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pWT065c

Plasmid

#107913

Purpose

W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

(TCF-LEF)7-BE3-P2A-Luc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR15aPer

Plasmid

#21116

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p5426

Plasmid

#168187

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::dsred PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-EMX1+38

Plasmid

#140579

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA EMX1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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p5204

Plasmid

#168181

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PcypD::sfgfp (frameshift)

Expression

Bacterial

Mutation

frameshift sfGFP

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Per

Plasmid

#21118

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-DNMT1+38

Plasmid

#140578

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA DNMT1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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hsp70:GalT-RFP

Plasmid

#105966

Purpose

heat shock inducible transgenesis, Galactsyl transferase (trans-golgi marker)

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

GalT

Tags

mRFP1

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10ΔDDE

Plasmid

#24552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty delta DDE

Expression

Bacterial

Mutation

removes 322 bp of the transposase-coding region

Promoter

CMV

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-3

Plasmid

#27107

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

TetO promoter

Use

Entry vector

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-R615A

Plasmid

#135995

Purpose

R615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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